Article
An efficient strategy for producing a stable, replaceable, highly efficient transgene expression system in silkworm, Bombyx mori.
Scientific reports - 5 Mar 2015
Long Dingpei, Lu Weijian, Zhang Yuli, Bi Lihui, Xiang Zhonghuai, Zhao Aichun
Abstract excerpt
We developed an efficient strategy that combines a method for the post-integration elimination of all transposon sequences, a site-specific recombination system, and an optimized fibroin H-chain expression system to produce a stable, replaceable, highly efficient transgene expression system in the silkworm (Bombyx mori) that overcomes the disadvantages of random insertion and post-integration instability of...
Topics
- Animals
- Animals, Genetically Modified
- Bombyx
- Crosses, Genetic
- DNA Transposable Elements
- Female
- Gene Expression
- Gene Order
- Gene Transfer Techniques
- Genes, Reporter
- Genetic Vectors
- Genomic Instability
- Homologous Recombination
- Male