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Allele-specific real-time polymerase chain reaction as a tool for urate transporter 1 mutation detection.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Makanga Juliet O, Christianto Antonius, Inazu Tetsuya

Abstract excerpt

Allele-specific polymerase chain reaction (ASPCR) method has long been applied for the detection of nucleotide variations and genotyping, which are detected by the presence or absence of DNA amplification PCR products. Recently, Real-Time PCR genotyping has fast developed and offered a rapid method of detecting mutations without the need of gel electrophoresis as with ASPCR. Here, we describe an easy and rapid...

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PMID: 25697655
DOI: 10.1007/978-1-4939-2365-6_8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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