Article
Tomato TILLING technology: development of a reverse genetics tool for the efficient isolation of mutants from Micro-Tom mutant libraries.
Plant & cell physiology - 1 Nov 2011
Okabe Yoshihiro, Asamizu Erika, Saito Takeshi, Matsukura Chiaki, Ariizumi Tohru, Brès Cécile, Rothan Christophe, Mizoguchi Tsuyoshi, Ezura Hiroshi
Abstract excerpt
To accelerate functional genomic research in tomato, we developed a Micro-Tom TILLING (Targeting Induced Local Lesions In Genomes) platform. DNA pools were constructed from 3,052 ethyl methanesulfonate (EMS) mutant lines treated with 0.5 or 1.0% EMS. The mutation frequency was calculated by screening 10 genes. The 0.5% EMS population had a mild mutation frequency of one mutation per 1,710 kb, whereas the 1.0% EMS...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.