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Article

Structural and catalytic effects of proline substitution and surface loop deletion in the extended active site of human carbonic anhydrase II.

The FEBS journal - 1 Apr 2015

Boone Christopher D, Rasi Valerio, Tu Chingkuang, McKenna Robert

Abstract excerpt

UNLABELLED: Bioengineering of a thermophilic enzyme starting from a mesophilic scaffold has proven to be a significant challenge, as several stabilizing elements have been proposed to be the foundation of thermal stability, including disulfide bridges, surface loop reduction, ionic pair networks, proline substitutions and aromatic clusters. This study emphasizes the effect of increasing the rigidity of human...

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Identifiers

PMID: 25683338
DOI: 10.1111/febs.13232
PMCID: PMC4400229

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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