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Article

Dramatic stabilization of the native state of human carbonic anhydrase II by an engineered disulfide bond.

Biochemistry - 31 Dec 2002

Mårtensson Lars-Göran, Karlsson Martin, Carlsson Uno

Abstract excerpt

To find a disulfide pair that could stabilize the enzyme human carbonic anhydrase II (HCA II), we grafted the disulfide bridge from the related and unusually stable carbonic anhydrase form from Neisseria gonorrhoeae (NGCA) into the human enzyme. Thus, the two Cys residues at positions 23 and 203 were engineered into a pseudo-wild-type form of HCA II (C206S), giving the mutant C206S/A23C/L203C. The disulfide bond...

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PMID: 12501217
DOI: 10.1021/bi020433+

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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