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Article

An intermolecular electrostatic interaction controls the prepore-to-pore transition in a cholesterol-dependent cytolysin.

Proceedings of the National Academy of Sciences of the United States of America - 17 Feb 2015

Wade Kristin R, Hotze Eileen M, Kuiper Michael J, Morton Craig J, Parker Michael W, Tweten Rodney K

Abstract excerpt

β-Barrel pore-forming toxins (βPFTs) form an obligatory oligomeric prepore intermediate before the formation of the β-barrel pore. The molecular components that control the critical prepore-to-pore transition remain unknown for βPFTs. Using the archetype βPFT perfringolysin O, we show that E183 of each monomer within the prepore complex forms an intermolecular electrostatic interaction with K336 of the adjacent...

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Identifiers

PMID: 25646411
DOI: 10.1073/pnas.1423754112
PMCID: PMC4343107

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Source receipt: pubmed, retrieved 7 Sept 2026.

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