Article
Comparison of next-generation sequencing mutation profiling with BRAF and IDH1 mutation-specific immunohistochemistry.
The American journal of surgical pathology - 1 Apr 2015
Jabbar Kausar J, Luthra Rajalakshmi, Patel Keyur P, Singh Rajesh R, Goswami Rashmi, Aldape Ken D, Medeiros L Jeffrey, Routbort Mark J
Abstract excerpt
Mutation-specific antibodies for BRAF V600E and IDH1 R132H offer convenient immunohistochemical (IHC) assays to detect these mutations in tumors. Previous studies using these antibodies have shown high sensitivity and specificity, but use in routine diagnosis with qualitative assessment has not b...
Topics
- Adult
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
- DNA Mutational Analysis
- False Negative Reactions
- Female
- High-Throughput Nucleotide Sequencing
- Humans
- Immunohistochemistry
- Isocitrate Dehydrogenase
- Male
- Middle Aged
- Mutation
- Neoplasms