Validation of a Manual Protocol for BRAF V600E Mutation-specific Immunohistochemistry.

doi:10.1097/PAI.0000000000000092

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Article

Validation of a Manual Protocol for BRAF V600E Mutation-specific Immunohistochemistry.

Applied immunohistochemistry & molecular morphology : AIMM - 1 Jan 2000

Dinges Hanns C, Capper David, Ritz Olga, Brüderlein Silke, Marienfeld Ralf, von Deimling Andreas, Möller Peter, Lennerz Jochen K

Abstract excerpt

Detection of BRAF V600E has diagnostic, prognostic, and therapeutic relevance. The recently developed BRAF V600E mutation-specific antibody has evolved into a feasible alternative to DNA analysis. The plethora of immunohistochemical protocols makes implementation tedious and, here we tested a set of manual and automated protocols and compared test performance with sequencing results. For assays, we employed...

Topics

Aspartic Acid
Automation, Laboratory
Biomarkers, Tumor
DNA Mutational Analysis
Decalcification Technique
Formaldehyde
Gene Expression
Humans
Immunohistochemistry
Leukemia, Hairy Cell

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Melanoma

Mutation

Proto-Oncogene Proteins B-raf

Tissue Embedding

Tissue Fixation

Valine

Source

Open in PubMed

Identifiers

PMID: 25611237
DOI: 10.1097/PAI.0000000000000092

Genes

BRAF

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-25611237-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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