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Article

Validation of a Manual Protocol for BRAF V600E Mutation-specific Immunohistochemistry.

Applied immunohistochemistry & molecular morphology : AIMM - 1 Jan 2000

Dinges Hanns C, Capper David, Ritz Olga, Brüderlein Silke, Marienfeld Ralf, von Deimling Andreas, Möller Peter, Lennerz Jochen K

Abstract excerpt

Detection of BRAF V600E has diagnostic, prognostic, and therapeutic relevance. The recently developed BRAF V600E mutation-specific antibody has evolved into a feasible alternative to DNA analysis. The plethora of immunohistochemical protocols makes implementation tedious and, here we tested a set of manual and automated protocols and compared test performance with sequencing results. For assays, we employed...

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Identifiers

PMID: 25611237
DOI: 10.1097/PAI.0000000000000092

Genes

BRAF

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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