Article
Detection of BRAF p.V600E mutations in melanomas: comparison of four methods argues for sequential use of immunohistochemistry and pyrosequencing.
The Journal of molecular diagnostics : JMD - 1 Jan 2013
Colomba Emeline, Hélias-Rodzewicz Zofia, Von Deimling Andreas, Marin Cristi, Terrones Nathalie, Pechaud Dominique, Surel Sylvie, Côté Jean-François, Peschaud Frédérique, Capper David, Blons Hélène, Zimmermann Ute, Clerici Thierry, Saiag Philippe, Emile Jean-François
Abstract excerpt
BRAF p.V600 mutation detection recently became necessary to treat metastatic melanoma patients with vemurafenib. This study compares different methods of detection of BRAF mutations. Melanoma samples from 111 patients were analyzed for BRAF mutations, and for 89 of them, results were obtained with the four following methods: Sanger sequencing, real-time PCR, immunohistochemistry, and pyrosequencing. All samples...
Topics
- DNA Mutational Analysis
- Humans
- Immunohistochemistry
- Indoles
- Melanoma
- Mutation
- Proto-Oncogene Proteins B-raf
- Real-Time Polymerase Chain Reaction
- Receptor, ErbB-2