Article
Using surface-enhanced Raman spectroscopy and electrochemically driven melting to discriminate Yersinia pestis from Y. pseudotuberculosis based on single nucleotide polymorphisms within unpurified polymerase chain reaction amplicons.
Analytical chemistry - 3 Feb 2015
Papadopoulou Evanthia, Goodchild Sarah A, Cleary David W, Weller Simon A, Gale Nittaya, Stubberfield Michael R, Brown Tom, Bartlett Philip N
Abstract excerpt
The development of sensors for the detection of pathogen-specific DNA, including relevant species/strain level discrimination, is critical in molecular diagnostics with major impacts in areas such as bioterrorism and food safety. Herein, we use electrochemically driven denaturation assays monitored by surface-enhanced Raman spectroscopy (SERS) to target single nucleotide polymorphisms (SNPs) that distinguish DNA...
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