Article
Enhanced arachidonic acid production from Mortierella alpina combining atmospheric and room temperature plasma (ARTP) and diethyl sulfate treatments.
Bioresource technology - 1 Feb 2015
Li Xiangyu, Liu Ruijie, Li Jing, Chang Ming, Liu Yuanfa, Jin Qingzhe, Wang Xingguo
Abstract excerpt
To obtain mutant strains with higher arachidonic acid (ARA) yields, the oleaginous fungus Mortierella alpina was mutated using atmospheric and room temperature plasma (ARTP) coupled with diethyl sulfate (DES). A visual compound filter operation was used in which a screening medium was supplemented with cerulenin, an inhibitor of fatty acid synthase (FAS), and triphenyltetrazolium chloride (TTC). The mutant strain...
Topics
- Arachidonic Acid
- Atmosphere
- Batch Cell Culture Techniques
- Biomass
- Ceruletide
- Fermentation
- Hydrogen-Ion Concentration
- Mortierella
- Mutagenesis
- Mutation
- Nitrogen