Article
[Operating conditions for the rapid mutation of the oleaginous yeast by atmospheric and room temperature plasmas and the characteristics of the mutants].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Mar 2011
Jin Lihua, Fang Mingyue, Zhang Chong, Jiang Peixia, Ge Nan, Li Heping, Xing Xinhui, Bao Chengyu
Abstract excerpt
To obtain oleaginous yeast mutants with improved lipid production and growth rates, an atmospheric and room temperature plasma (ARTP) jet was used with a 96-well plate for high throughput screening. Mutants with changes in growth rates and lipid contents were obtained. At a lethality rate of 99%, the positive mutation rate of the yeast cells was 27.2% evaluated by the growth rates of the mutants and the...
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