Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A method for using direct injection of plasmid DNA to study cis-regulatory element activity in F0 Xenopus embryos and tadpoles.

Developmental biology - 1 Feb 2015

Wang Chen, Szaro Ben G

Abstract excerpt

The ability to express exogenous reporter genes in intact, externally developing embryos, such as Xenopus, is a powerful tool for characterizing the activity of cis-regulatory gene elements during development. Although methods exist for generating transgenic Xenopus lines, more simplified methods for use with F0 animals would significantly speed the characterization of these elements. We discovered that injecting...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Genes, Reporter
Share to Genetic Techniques
Share to Green Fluorescent Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Integrases

Share to Plasmids

Share to Regulatory Sequences, Nucleic Acid

Share to Xenopus laevis

Source

Identifiers

PMID: 25448690
DOI: 10.1016/j.ydbio.2014.11.010

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A method for using direct injection of plasmid DNA to study cis-regulatory element activity in F0 Xenopus embryos and tadpoles.PMID 25448690Read ↗

Chicken beta-globin insulator overcomes variegation of transgenes in Xenopus embryos.Semantic proximity Read ↗

Targeted gene expression in transgenic Xenopus using the binary Gal4-UAS system.Semantic proximity Read ↗

Effective gene trapping mediated by Sleeping Beauty transposon.Semantic proximity Read ↗

Stable germline transgenesis using the <i>Minos</i> Tc1/ <i>mariner</i> element in the sea urchin, <i>Lytechinus pictus</i>Semantic proximity Read ↗

Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos.Semantic proximity Read ↗

Application of Transposon Systems in The Transgenesis of Bovine Somatic And Germ CellsSemantic proximity Read ↗

Heterologous reporter expression in the planarian <i>Schmidtea mediterranea</i> through somatic mRNA transfectionSemantic proximity Read ↗

Inducible gene expression in transgenic Xenopus embryos.Semantic proximity Read ↗

Quantitative comparison of cis-regulatory element (CRE) activities in transgenic Drosophila melanogaster.Semantic proximity Read ↗

Inducible control of tissue-specific transgene expression in Xenopus tropicalis transgenic lines.Semantic proximity Read ↗

Transposon tools for recombinant DNA manipulation: characterization of transcriptional regulators from yeast, Xenopus, and mouse.Semantic proximity Read ↗

Optimized Lentiviral Vector Design Improves Titer and Transgene Expression of Vectors Containing the Chicken β-Globin Locus HS4 Insulator ElementSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.