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Dramatic improvement in genome assembly achieved using doubled-haploid genomes.

Scientific reports - 27 Oct 2014

Zhang Hong, Tan Engkong, Suzuki Yutaka, Hirose Yusuke, Kinoshita Shigeharu, Okano Hideyuki, Kudoh Jun, Shimizu Atsushi, Saito Kazuyoshi, Watabe Shugo, Asakawa Shuichi

Abstract excerpt

Improvement in de novo assembly of large genomes is still to be desired. Here, we improved draft genome sequence quality by employing doubled-haploid individuals. We sequenced wildtype and doubled-haploid Takifugu rubripes genomes, under the same conditions, using the Illumina platform and assembled contigs with SOAPdenovo2. We observed 5.4-fold and 2.6-fold improvement in the sizes of the N50 contig and scaffold...

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Identifiers

PMID: 25345569
DOI: 10.1038/srep06780
PMCID: PMC5381364

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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