Article
A natural heme-signature variant of CYP267A1 from Sorangium cellulosum So ce56 executes diverse ω-hydroxylation.
The FEBS journal - 1 Jan 2015
Khatri Yogan, Hannemann Frank, Girhard Marco, Kappl Reinhard, Hutter Michael, Urlacher Vlada B, Bernhardt Rita
Abstract excerpt
A novel naturally occurring heme-signature variant of CYP267A1 from myxobacterium Sorangium cellulosum So ce56 and its mutant L366F, the actual mimic of the 'conserved' heme-signature of cytochromes P450, were heterologously expressed in Escherichia coli in a soluble form and purified. The UV-visible characteristics of both variants were highly similar. Although leucine replaced the phenylalanine in the...
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