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Article

Rescue of the highly virulent classical swine fever virus strain "Koslov" from cloned cDNA and first insights into genome variations relevant for virulence.

Virology - 1 Nov 2014

Fahnøe Ulrik, Pedersen Anders Gorm, Risager Peter Christian, Nielsen Jens, Belsham Graham J, Höper Dirk, Beer Martin, Rasmussen Thomas Bruun

Abstract excerpt

Classical swine fever virus (CSFV) strain "Koslov" is highly virulent with a mortality rate of up to 100% in pigs. In this study, we modified non-functional cDNAs generated from the blood of Koslov virus infected pigs by site-directed mutagenesis, removing non-synonymous mutations step-by-step, thereby producing genomes encoding the consensus amino acid sequence. Viruses rescued from the construct corresponding...

Topics

Animals
Cell Line
Classical Swine Fever
Classical Swine Fever Virus
Cloning, Molecular
DNA, Complementary
Genetic Variation
Genome, Viral
Kidney
Swine

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Time Factors

Virulence

Source

Identifiers

PMID: 25240324
DOI: 10.1016/j.virol.2014.08.021

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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