Article
Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping.
BMC genomics - 9 Aug 2018
Johnston Camille Melissa, Fahnøe Ulrik, Belsham Graham J, Rasmussen Thomas Bruun
Abstract excerpt
BACKGROUND: Direct molecular cloning of full-length cDNAs derived from viral RNA is an approach to identify the individual viral genomes within a virus population. This enables characterization of distinct viral haplotypes present during infection. RESULTS: In this study, we recover individual genomes of classical swine fever virus (CSFV), present in a pig infected with vKos that was rescued from a cDNA clone...
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