Article
Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. I. Initial comparison of four protocols.
Diseases of aquatic organisms - 21 Aug 2014
Warg Janet V, Clement Travis, Cornwell Emily R, Cruz Angela, Getchell Rodman G, Giray Cem, Goodwin Andrew E, Groocock Geoffrey H, Faisal Mohamed, Kim Robert, Merry Gwenn E, Phelps Nicholas B D, Reising Monica M, Standish Isaac, Zhang Yan, Toohey-Kurth Kathy
Abstract excerpt
Eight laboratories worked collectively to evaluate 4 real-time RT-PCR (rRT-PCR) protocols targeting viral hemorrhagic septicemia virus (VHSV) being considered for deployment to a USA laboratory testing network. The protocols utilized previously published primers and probe sets developed for detection and surveillance of VHSV. All participating laboratories received and followed a standard operating protocol for...
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