Article
A novel multiplex RT-qPCR method based on dual-labelled probes suitable for typing all known genotypes of viral haemorrhagic septicaemia virus.
Journal of fish diseases - 1 Apr 2016
Vázquez D, López-Vázquez C, Skall H F, Mikkelsen S S, Olesen N J, Dopazo C P
Abstract excerpt
Viral haemorrhagic septicaemia (VHS) is a notifiable fish disease, whose causative agent is a rhabdovirus isolated from a wide range of fish species, not only in fresh but also in marine and brackish waters. Phylogenetic studies have identified four major genotypes, with a strong geographical relationship. In this study, we have designed and validated a new procedure--named binary multiplex RT-qPCR...
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