Article
Direct sequencing of DNA pooling for screening highly informative SNPs in dairy cattle.
Yi chuan = Hereditas - 1 Jul 2014
Chu Qin, Li Dong, Hou Shiyu, Shi Wanhai, Liu Lin, Wang Yachun
Abstract excerpt
In this study, 139 bovine single nuclear polymorphisms (SNPs) were firstly selected and then directly sequenced using DNA pooling. Based on the ratio of two signal peak values, 92 SNPs with the ratio over 1/2 were considered as potential highly informative markers. To further verify the reliability of screening system, 59 SNP markers were genotyped in 122 Holstein cattle using matrix-assisted laser...
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