Article
Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
Journal of proteome research - 5 Mar 2010
Hempel Kristina, Pané-Farré Jan, Otto Andreas, Sievers Susanne, Hecker Michael, Becher Dörte
Abstract excerpt
Most of the Staphylococcus aureus virulence factors are either cell surface exposed or secreted. Here we report a global and quantitative analysis of staphylococcal cell surface-associated proteins using a combination of (14)N(15)N metabolic labeling, biotinylation, and GeLC-MS/MS. To address the...
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