Article
HNF-4α regulates expression of human ornithin carbamoyltransferase through interaction with two positive cis-acting regulatory elements located in the proximal promoter.
Folia biologica - 1 Jan 2014
Lukšan O, Dvořáková L, Jirsa M
Abstract excerpt
OTC encodes ornithine carbamoyltransferase, mitochondrial matrix enzyme involved in the synthesis of urea. The tissue-specific expression of OTC in the liver and intestine is dependent on the interaction of OTC promoter with an upstream enhancer. HNF-4 and C/EBPβ are crucial for this interaction in the rat and mouse. In the present study we focused on characterization of elements involved in the regulation of OTC...
Topics
- 5' Flanking Region
- Animals
- Base Sequence
- Computer Simulation
- DNA
- DNA Footprinting
- Deoxyribonuclease I
- Electrophoretic Mobility Shift Assay
- Gene Expression Regulation, Enzymologic
- Genes, Reporter
- Hep G2 Cells
- Hepatocyte Nuclear Factor 4
- Humans