Article
Identification of the translational start site of codon-optimized mCherry in Mycobacterium tuberculosis.
BMC research notes - 17 Jun 2014
Carroll Paul, Muwanguzi-Karugaba Julian, Melief Eduard, Files Megan, Parish Tanya
Abstract excerpt
BACKGROUND: Fluorescent proteins are used widely as reporter genes in many organisms. We previously codon-optimized mCherry for Mycobacterium tuberculosis and generated expression constructs with high level expression in mycobacteria with multiple uses in vitro and in vivo. However, little is known about the expression of fluorescent proteins in mycobacteria and the translational start codon for mCherry has not...
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