Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Codon-optimized fluorescent mTFP and mCherry for microscopic visualization and genetic counterselection of streptococci and enterococci.

Journal of microbiological methods - 1 Sept 2015

Vickerman M Margaret, Mansfield Jillian M, Zhu Min, Walters Katherine S, Banas Jeffrey A

Abstract excerpt

Despite the powerful potential of fluorescent proteins for labeling bacteria, their use has been limited in multi-species oral biofilm models. Fermentative metabolism by streptococcal species that initiate biofilm colonization results in an acidic, reduced microenvironment that may limit the activities of some fluorescent proteins which are influenced by pH and oxygen availability. The need to reliably...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biofilms
Share to Enterococcus

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Fluorescent Dyes

Share to Hydrogen-Ion Concentration

Share to Luminescent Proteins

Share to Microscopy, Fluorescence

Share to Red Fluorescent Protein

Share to Streptococcus

Source

Open in PubMed Open full text

Identifiers

PMID: 26122309
DOI: 10.1016/j.mimet.2015.06.010
PMCID: PMC4522221

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Codon-optimized fluorescent mTFP and mCherry for microscopic visualization and genetic counterselection of streptococci and enterococci.PMID 26122309Read ↗

Design, Development and Validation of New Fluorescent Strains for Studying Oral StreptococciSemantic proximity Read ↗

Fluorescence Tools Adapted for Real-Time Monitoring of the Behaviors of Streptococcus SpeciesSemantic proximity Read ↗

A Modular Genetic Toolbox for Precise Gene Regulation and Multi-Color Imaging in StreptococciSemantic proximity Read ↗

Stable Expression of Modified Green Fluorescent Protein in Group B Streptococci To Enable Visualization in Experimental SystemsSemantic proximity Read ↗

The development of fluorescent protein tracing vectors for multicolor imaging of clinically isolated Staphylococcus aureus.Semantic proximity Read ↗

Toolbox for fluorescent labelling of <i>Pseudomonas aeruginosa</i> across scales: from single cells to bacterial communities and host infection modelsSemantic proximity Read ↗

The green fluorescent protein as a visible marker for lactic acid bacteria in complex ecosystemsSemantic proximity Read ↗

Seeing red; the development of pON.mCherry, a broad-host range constitutive expression plasmid for Gram-negative bacteria.Semantic proximity Read ↗

Adaptation of Prokaryotic Toxins for Negative Selection and Cloning-Independent Markerless Mutagenesis in Streptococcus Species.Semantic proximity Read ↗

Microbiome imaging goes à la carte: Incorporating click chemistry into the fluorescence-activating and absorption-shifting tag (FAST) imaging platformSemantic proximity Read ↗

investigating acid production by Streptococcus mutans with a surface-displayed pH-sensitive green fluorescent protein.Semantic proximity Read ↗

Development of SacB-based counterselection for efficient allelic exchange in Fusobacterium nucleatumSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.