Article
Complete pyridine-nucleotide-specific conversion of an NADH-dependent ferredoxin reductase.
The Biochemical journal - 1 Sept 2014
Nishizawa Akito, Harada Ayaka, Senda Miki, Tachihara Yuka, Muramatsu Daisuke, Kishigami Shinya, Mori Shigemasa, Sugiyama Keisuke, Senda Toshiya, Kimura Shigenobu
Abstract excerpt
The coenzyme specificity of enzymes is one of the critical parameters for the engineered production of biological compounds using bacteria. Since NADPH is produced abundantly in photosynthetic organisms, conversion of an NADH-specific enzyme into an NADPH-specific one is a useful approach for the efficient carbon-neutral production of biological compounds in photosynthetic organisms. In the present study, an...
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