Article
Development and application of quantitative detection method for viral hemorrhagic septicemia virus (VHSV) genogroup IVa.
Viruses - 23 May 2014
Kim Jong-Oh, Kim Wi-Sik, Kim Si-Woo, Han Hyun-Ja, Kim Jin Woo, Park Myoung Ae, Oh Myung-Joo
Abstract excerpt
Viral hemorrhagic septicemia virus (VHSV) is a problematic pathogen in olive flounder (Paralichthys olivaceus) aquaculture farms in Korea. Thus, it is necessary to develop a rapid and accurate diagnostic method to detect this virus. We developed a quantitative RT-PCR (qRT-PCR) method based on the nucleocapsid (N) gene sequence of Korean VHSV isolate (Genogroup IVa). The slope and R² values of the primer set...
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