Article
An efficient protocol for the generation of monocyte derived dendritic cells using serum-free media for clinical applications in post remission AML patients.
Annals of clinical and laboratory science - 1 Jan 2014
da Silva Simoneti Gisele, Saad Sara Teresinha Olalla, Gilli Simone Cristina Olenscki
Abstract excerpt
Protocols for the generation of dendritic cells (DCs) using serum as a supplementation of culture media leads to reactions due to animal proteins and disease transmissions. Several types of serum-free media (SFM), based on "good manufacture practices" (GMP), have recently been used and seem to be a viable option. The aim of this study was to evaluate the results of the differentiation, maturation, and function of...
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