Article
Establishment of serum-free pre-colony forming unit assays for differentiation of primitive hematopoietic progenitors: serum induces early macrophage differentiation and inhibits early erythroid differentiation of CD34++CD38- cells.
Annals of hematology - 1 Jan 2001
Willems R, Henckaerts E, Lenjou M, Nijs G, Rodrigus I, Moulijn A C, Slegers H, Berneman Z N, Van Bockstaele D R
Abstract excerpt
In this report we show that serum has differentiation-inducing effects on primitive hematopoietic progenitor cells with the CD34++CD38- immunophenotype. Using the pre-colony forming unit (pre-CFU) assay as a model for early myelopoiesis, we compared the effects of serum-containing and serum-free media and evaluated different cytokine cocktails [interleukin (IL)-1, IL-3, IL-6, kit ligand with and without the...
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