Article
Allosteric transition induced by Mg²⁺ ion in a transactivator monitored by SERS.
The journal of physical chemistry. B - 22 May 2014
Kundu Partha P, Bhowmick Tuhin, Swapna Ganduri, Pavan Kumar G V, Nagaraja Valakunja, Narayana Chandrabhas
Abstract excerpt
We demonstrate the utility of the surface-enhanced Raman spectroscopy (SERS) to monitor conformational transitions in protein upon ligand binding. The changes in protein's secondary and tertiary structures were monitored using amide and aliphatic/aromatic side chain vibrations. Changes in these bands are suggestive of the stabilization of the secondary and tertiary structure of transcription activator protein C...
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