Article
Development of genic SSR markers from transcriptome sequencing of pear buds.
Journal of Zhejiang University. Science. B - 1 Apr 2014
Yue Xiao-yan, Liu Guo-qin, Zong Yu, Teng Yuan-wen, Cai Dan-ying
Abstract excerpt
A total of 8375 genic simple sequence repeat (SSR) loci were discovered from a unigene set assembled from 116282 transcriptomic unigenes in this study. Dinucleotide repeat motifs were the most common with a frequency of 65.11%, followed by trinucleotide (32.81%). A total of 4100 primer pairs were designed from the SSR loci. Of these, 343 primer pairs (repeat length ≥15 bp) were synthesized with an M13 tail and...
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