Article
De novo assembly, functional annotation, and marker development of Asian pear (Pyrus pyrifolia) fruit transcriptome through massively parallel sequencing.
Genetics and molecular research : GMR - 28 Dec 2015
Li J F, Gao Z, Lou Y S, Luo M, Song S R, Xu W P, Wang S P, Zhang C X
Abstract excerpt
This study investigated the Asian pear transcriptome using the RNA-Seq normalized fruit cDNA library to create a transcriptomic resource for unigene and marker discovery. Following the removal of lowquality reads, 127,085,054 trimmed reads were assembled de novo to yield 37,649 non-redundant unigenes with an average length of 599 bp. Alternative splicing events were detected in 4121 contigs. A total of 30,560...
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