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Analysis of EST-SSR characteristics of prickly ash transcriptome and development of molecular markers.

Scientific reports - 10 Dec 2025

Liu Jiamin, Zhuo Siyu, Xiang Shimin, Du Jin, Zhang Ruhan, Tian Ailin, Liu Linyu, Cai Xinyi, Ma Zhixin, Ren Yun, Chen Zexiong

Abstract excerpt

To explore the genetic diversity and relationships among prickly ash germplasm resources in China, the number, distribution, and repeat types of simple sequence repeats (EST-SSR) loci were analyzed using the MISA software system based on the transcriptome data of prickly ash. Thirty-five prickly ash germplasms from nine provinces were selected, and 30 pairs of EST-SSR primers were randomly designed for...

Topics

Microsatellite Repeats
Expressed Sequence Tags
Transcriptome
Genetic Markers
Polymorphism, Genetic
China
Genetic Variation

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 41372287
DOI: 10.1038/s41598-025-27550-4
PMCID: PMC12695933

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