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Article

SSR Loci Analysis in Transcriptome and Molecular Marker Development in Polygonatum sibiricum.

BioMed research international - 1 Jan 2022

Yang Qingwen, Jiang Yujie, Wang Yuping, Han Ruilian, Liang Zongsuo, He Qiuling, Jia Qiaojun

Abstract excerpt

To study the SSR loci information and develop molecular markers, a total of 435,858 unigenes in transcriptome of Polygonatum sibiricum were used to explore SSR. The distribution frequency of SSR and the basic characteristics of repeat motifs were analyzed using MISA software, and SSR primers were designed by Primer 3.0 software and then validated by PCR. Moreover, the gene function analysis of SSR Unigene was...

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Identifiers

PMID: 35299892
DOI: 10.1155/2022/4237913
PMCID: PMC8923796

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