Article
Mining and characterizing microsatellites from citrus ESTs.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 May 2006
Chen Chunxian, Zhou Ping, Choi Young A, Huang Shu, Gmitter Fred G
Abstract excerpt
Freely available computer programs were arranged in a pipeline to extract microsatellites from public citrus EST sequences, retrieved from the NCBI. In total, 3,278 bi- to hexa-type SSR-containing sequences were identified from 56,199 citrus ESTs. On an average, one SSR was found per 5.2 kb of EST sequence, with the tri-nucleotide motifs as the most abundant. Primer sequences flanking SSR motifs were successfully...
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