Article
Generation and characterization of a tomato DCL3-silencing mutant.
Plant science : an international journal of experimental plant biology - 1 May 2014
Kravchik Michael, Damodharan Subha, Stav Ran, Arazi Tzahi
Abstract excerpt
DICER-like 3 (DCL3) is a major player in heterochromatic 24-nucleotide (nt) small RNA (sRNA) and long microRNA (lmiRNA) biogenesis, and higher plant DCL3 mutants have been characterized from Arabidopsis thaliana and rice. Here, a tomato DCL3 (SlDCL3) mutant was generated through the use of trans-activated artificial miRNA and characterized. Constitutive trans-activation knocked down SlDCL3 levels by ∼64%,...
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