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Generation and characterization of a tomato DCL3-silencing mutant.

Plant science : an international journal of experimental plant biology - 1 May 2014

Kravchik Michael, Damodharan Subha, Stav Ran, Arazi Tzahi

Abstract excerpt

DICER-like 3 (DCL3) is a major player in heterochromatic 24-nucleotide (nt) small RNA (sRNA) and long microRNA (lmiRNA) biogenesis, and higher plant DCL3 mutants have been characterized from Arabidopsis thaliana and rice. Here, a tomato DCL3 (SlDCL3) mutant was generated through the use of trans-activated artificial miRNA and characterized. Constitutive trans-activation knocked down SlDCL3 levels by ∼64%,...

Topics

Gene Expression Regulation, Plant
Gene Silencing
Solanum lycopersicum
MicroRNAs
Molecular Sequence Data
Mutation
Plant Proteins
Protein Structure, Secondary
Ribonuclease III
Seedlings
Sequence Analysis, Protein

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 24656338
DOI: 10.1016/j.plantsci.2014.02.007

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