Evaluation of saliva as a source of human DNA for population and association studies.

doi:10.1016/j.ab.2006.03.021

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Article

Evaluation of saliva as a source of human DNA for population and association studies.

Analytical biochemistry - 15 Jun 2006

Quinque Dominique, Kittler Ralf, Kayser Manfred, Stoneking Mark, Nasidze Ivan

Abstract excerpt

A simple noninvasive procedure for saliva sample collection and DNA extraction was developed. On average, the amount of human DNA (as measured by a TaqMan-based assay) was about 11.4 microg/mL saliva, which is more than can be obtained from other noninvasive samples such as cheek swabs. However, the presence of large amounts of nonhuman DNA (up to 90% of the total extracted DNA) in saliva samples does necessitate...

Topics

Base Sequence
DNA
Female
Genetic Testing
Genotype
Humans
Male
Molecular Sequence Data
Saliva
Specimen Handling
Time Factors

Source

Open in PubMed

Identifiers

PMID: 16620753
DOI: 10.1016/j.ab.2006.03.021

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