Article
Molecular method development and establishment of a database for clinical and epidemiological herpes simplex virus 1 strain comparisons.
Journal of clinical microbiology - 1 May 2014
Dean Amy B, St George Kirsten
Abstract excerpt
Previous methods of herpes simplex virus 1 (HSV-1) genotype analysis have lacked sufficient discriminatory power for strain analysis within genotypes. The hypervariable reiterative repeat regions in the US1 and US12 introns, known as ReIV, were targeted for strain comparison. PCR methods for these extremely GC-rich target regions were optimized to give reproducible amplicons that were visualized by capillary...
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