Article
Zinc in lipase L1 from Geobacillus stearothermophilus L1 and structural implications on thermal stability.
FEBS letters - 20 Jun 2005
Choi Won-Chan, Kim Myung Hee, Ro Hyeon-Su, Ryu Sang Ryeol, Oh Tae-Kwang, Lee Jung-Kee
Abstract excerpt
Lipase L1 from Geobacillus stearothermophilus L1 contains an unusual extra domain, making a tight intramolecular interaction with the main catalytic domain through a Zn2+-binding coordination. To elucidate the role of the Zn2+, we disrupted the Zn2+-binding site by mutating the zinc-ligand residu...
Topics
- Amino Acid Sequence
- Bacillaceae
- Binding Sites
- Catalytic Domain
- Enzyme Stability
- Lipase
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Denaturation
- Protein Structure, Tertiary
- Temperature
- Zinc