Article
Sequencing, assembling, and correcting draft genomes using recombinant populations.
G3 (Bethesda, Md.) - 16 Apr 2014
Hahn Matthew W, Zhang Simo V, Moyle Leonie C
Abstract excerpt
Current de novo whole-genome sequencing approaches often are inadequate for organisms lacking substantial preexisting genetic data. Problems with these methods are manifest as: large numbers of scaffolds that are not ordered within chromosomes or assigned to individual chromosomes, misassembly of allelic sequences as separate loci when the individual(s) being sequenced are heterozygous, and the collapse of...
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