Article
Nanoparticle imaging and diagnostic of Caenorhabditis elegans intracellular pH.
Analytical biochemistry - 1 Apr 2014
Mathew Neal D, Mathew Mark D, Surawski Peter P T
Abstract excerpt
A novel diagnostic tool has been developed for the characterization of intracellular pH (pHi) in the model organism Caenorhabditis elegans. This tool exploits the chemical stability of colloidal silica and the pH sensitivity of certain fluorescent dyes. Once ingested, the fluorescent colloidal dispersion yields a reliable visual indication of pH without the use of chemical fixatives or damaging the nematode. The...
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