Article
Genotyping of clinical Mycobacterium tuberculosis isolates based on IS6110 and MIRU-VNTR polymorphisms.
BioMed research international - 1 Jan 2013
Żaczek Anna, Brzostek Anna, Wojtasik Arkadiusz, Dziadek Jarosław, Sajduda Anna
Abstract excerpt
In this study, 155 clinical Mycobacterium tuberculosis isolates were subject to genotyping with fast ligation-mediated PCR (FLiP). This typing method is a modified mixed-linker PCR, a rapid approach based on the PCR amplification of HhaI restriction fragments of genomic DNA containing the 3' end of IS6110 and resolving the amplicons by polyacrylamide gel electrophoresis. The results were compared with previous...
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