Prospective universal application of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping to characterize Mycobacterium tuberculosis isolates for fast identification of clustered and orphan cases.

doi:10.1128/JCM.02308-08

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Article

Prospective universal application of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat genotyping to characterize Mycobacterium tuberculosis isolates for fast identification of clustered and orphan cases.

Journal of clinical microbiology - 1 Jul 2009

Alonso-Rodriguez Noelia, Martínez-Lirola Miguel, Sánchez M Luisa, Herranz Marta, Peñafiel Teresa, Bonillo Magdalena del Carmen, Gonzalez-Rivera Milagros, Martínez Juan, Cabezas Teresa, Diez-García Luis Felipe, Bouza Emilio, García de Viedma Darío

Abstract excerpt

The use of molecular tools for genotyping Mycobacterium tuberculosis isolates in epidemiological surveys in order to identify clustered and orphan strains requires faster response times than those offered by the reference method, IS6110 restriction fragment length polymorphism (RFLP) genotyping. A method based on PCR, the mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR)...

Topics

Bacterial Typing Techniques
Cluster Analysis
DNA Fingerprinting
DNA, Bacterial
Genotype
Humans
Interspersed Repetitive Sequences
Minisatellite Repeats

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Epidemiology

Mycobacterium tuberculosis

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Sensitivity and Specificity

Tuberculosis

Source

Open in PubMed Open full text

Identifiers

PMID: 19458183
DOI: 10.1128/JCM.02308-08
PMCID: PMC2708494

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-19458183-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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