Article
Application of FLiP method for differentiation of Mycobacterium tuberculosis strains in comparison to commonly used methods, spoligotyping and MIRU-VNTR typing.
Polish journal of microbiology - 1 Jan 2013
Zaczek Anna, Brzostek Anna, Górna Alina, Sajduda Anna, Wojtasik Arkadiusz, Dziadek Jarosław
Abstract excerpt
The current "gold standard" in molecular epidemiological studies of Mycobacterium tuberculosis is IS6110 RFLP based on IS6110 polymorphism. However PCR-based methods are becoming increasingly important. Recently, fast ligation-mediated PCR (FLiP), based on IS6110 polymorphism was proposed. In this study, the discriminatory power of FLIP, spoligotyping and MIRU-VNTR typing, in differentiation of M. tuberculosis...
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