Article
A 21-locus autosomal SNP multiplex and its application in forensic science.
Journal of forensic sciences - 1 Jan 2014
Hou Guangwei, Jiang Xianhua, Yang Yanyan, Jia Fei, Li Qiang, Zhao Jinling, Guo Fei, Liu Limin
Abstract excerpt
To develop a cost-effective technique for single-nucleotide polymorphism (SNP) genotyping and improve the efficiency to analyze degraded DNA, we have established a novel multiplex system including 21-locus autosomal SNPs and amelogenin locus, which was based on allele-specific amplification (ASA) and universal reporter primers (URP). The target amplicons for each of the 21 SNPs arranged from 63 base pair (bp) to...
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