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Expansion of a SNaPshot assay to a 55-SNP multiplex: Assay enhancements, validation, and power in forensic science.

Electrophoresis - 1 May 2016

Wang Qian, Fu Lihong, Zhang Xiaojing, Dai Xinyu, Bai Mei, Fu Guangping, Cong Bin, Li Shujin

Abstract excerpt

A previously developed multiplex assay with 44 individual identification SNPs was expanded to a 55plex assay. Fifty-four highly informative SNPs and an amelogenin sex marker were amplified in one PCR reaction and then detected with two SNaPshot reactions using CE. PCR primers for four loci, 28 single-base extension primers, and the reaction conditions were altered to improve the robustness of the method. A...

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PMID: 26864788
DOI: 10.1002/elps.201500353

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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