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Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.

Analytical biochemistry - 1 Feb 2016

Asari Masaru, Okuda Katsuhiro, Hoshina Chisato, Omura Tomohiro, Tasaki Yoshikazu, Shiono Hiroshi, Matsubara Kazuo, Shimizu Keiko

Abstract excerpt

The aim of this study was to develop a cost-effective genotyping method using high-quality DNA for human identification. A total of 21 short tandem repeats (STRs) and amelogenin were selected, and fluorescent fragments at 22 loci were simultaneously amplified in a single-tube reaction using locus-specific primers with 24-base universal tails and four fluorescent universal primers. Several nucleotide substitutions...

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PMID: 26505528
DOI: 10.1016/j.ab.2015.10.005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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