Article
[Study on functions of N-carbohydrate chains in human parainfluenza virus type 3 hemagglutinin-neuraminidase protein].
Bing du xue bao = Chinese journal of virology - 1 Sept 2013
Chu Fu-lu, Wen Hong-ling, Hou Gui-hua, Lin Bin, Zhang Wen-qiang, Song Yan-yan, Ren Gui-jie, Sun Cheng-xi, Li Zhen-mei, Wang Zhi-yu
Abstract excerpt
To determine the functions of N-carbohydrate chains in human parainfluenza virus type 3 hemagglutinin-neuraminidase(HN) protein, a PCR-based site-directed mutagenesis method was used to obtain N-glycan mutants. Protein electrophoresis rate, cell surface expression,receptor binding activity, neuraminidase activity and cell fusion promotion activity were determined. The HN proteins of single mutants (G1, G2, and...
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