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Article

Simple method for Shiga toxin 2e purification by affinity chromatography via binding to the divinyl sulfone group.

PloS one - 1 Jan 2013

Arimitsu Hideyuki, Sasaki Keiko, Kojima Hiroe, Yanaka Tadashi, Tsuji Takao

Abstract excerpt

Here we describe a simple affinity purification method for Shiga toxin 2e (Stx2e), a major causative factor of edema disease in swine. Escherichia coli strain MV1184 transformed with the expression plasmid pBSK-Stx2e produced Stx2e when cultivated in CAYE broth containing lincomycin. Stx2e bound to commercial D-galactose gel, containing α-D-galactose immobilized on agarose resin via a divinyl sulfone linker, and...

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Identifiers

PMID: 24340102
DOI: 10.1371/journal.pone.0083577
PMCID: PMC3858352

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