Article
Genetic and immunological analysis of a novel variant of Shiga toxin 1 from bovine Escherichia coli strains and development of bead-ELISA to detect the variant toxin.
Microbiology and immunology - 1 Jan 2003
Ohmura-Hoshino Mari, Ho Soon-Tae, Kurazono Hisao, Igarashi Kazuei, Yamasaki Shinji, Takeda Yoshifumi
Abstract excerpt
A novel variant of Shiga toxin 1 (Stx1) was identified from bovine Escherichia coli strains. The stx1 variant genes designated as stx1v51 and stx1v52 were cloned and sequenced. The two variant genes differed each other by 2 bp, but the deduced amino acid sequences of the two Stx1 variant toxins were the same and had 94% and 92% homology to that of prototype A and B subunits of Stx1, respectively. The variant...
Topics
- Amino Acid Sequence
- Animals
- Cattle
- Cattle Diseases
- Chlorocebus aethiops
- Cloning, Molecular
- Enzyme-Linked Immunosorbent Assay
- Escherichia coli
- Escherichia coli Infections
- Genetic Variation
- Immunodiffusion
- Mice
- Molecular Sequence Data