Article
Brain tumor cell line authentication, an efficient alternative to capillary electrophoresis by using a microfluidics-based system.
Neuro-oncology - 1 Jan 2014
An Qian, Fillmore Helen L, Vouri Mikaella, Pilkington Geoffrey J
Abstract excerpt
BACKGROUND: The current method for cell line authentication is genotyping based on short tandem repeat (STR)-PCR involving coamplification of a panel of STR loci by multiplex PCR and downstream fragment length analysis (FLA), usually performed by capillary electrophoresis. FLA by capillary electrophoresis is time-consuming and can be expensive, as the facilities are generally not accessible for many research...
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